The lines between Southampton and Portsmouth will be shut on Sunday.
Rail maintenance works will be getting underway throughout the day, forcing the stopping services between Southampton Central and Portsmouth to halt.
Railway lines between Eastleigh and Portsmouth will also be impacted.
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A spokesperson from South Western Railway said: “The morning and early afternoon London Waterloo to Poole/Portsmouth via Eastleigh services will not divide at Eastleigh. These services will continue to Poole only.
“The afternoon and evening London Waterloo to Portsmouth via Eastleigh services will terminate at Eastleigh.
“Buses will run between Eastleigh and Portsmouth via Fareham.
“Southampton Central to Portsmouth stopping services will not run.
“Buses will run between Southampton Central and Portsmouth via Fareham.”
Southampton City Council has urged travellers to plan ahead as a result of the disruption.